Part:BBa_K4757067:Design

Construct for sRNA testing with RBS 1

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1110
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 388
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 925
  Illegal SapI site found at 1255
  Illegal SapI.rc site found at 1229
  

Design Notes

Ps1/Ps2-XylS is on a different part of the plasmid. pEM7-RBS-mKate2-degradation tag was tested in reverse complement orientation.

Source

Gene-synthesis